Protein_Domain
PolyWb

Part:BBa_K792008:Design

Designed by: Manuel Giménez   Group: iGEM12_Buenos_Aires   (2012-09-25)


Tryptophan rich peptide (PolyWb)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PolyWb was desing taking into acount the following consideration:

  1. avoided repeating the same residue in tandem to minimize local tRNA depleation
  2. avoided Trp in tandem because of therir low solubility
  3. we included Gly to avoid the formation of stable structures
  4. included acidic and basic amino acids to increase solubility

Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.



Source

Designed by BsAs Team 2012

References